FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell48.35100000095596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell48.35100000095596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138635
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA95870.3054512550838183No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG58160.1853034838393123No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC54290.17297328297173772No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT49280.15701092991061402No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT44960.14324698475611214No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA40760.12986537141145754No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC39790.1267748559485254No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT39160.12476761394682721No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC36490.11626073117772535No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA34680.11049389304586228No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA33770.1075945434878538No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC33730.10746709955123804No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA32910.104854498850615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG36050.018.2869475
CCGATCT89600.018.2057614
TCCGATC90400.018.0695363
CGATCTA14950.017.9095485
TTCCGAT91400.017.8718382
CTTCCGA94550.017.4555171
GTACGAT1305.792899E-717.30699320
CGATCTT21450.017.0977575
TATACCG2650.016.9809886
CGGTACT11150.016.34541338
TATCCCG1257.637529E-616.19934710
CGGTACC8800.016.10730445
TTAACGG8850.016.01706735
CGAGGGT8300.015.9933316
GTATTAG18100.015.9228821
TAACGGC9000.015.75011636
ACGCGCC1004.170682E-415.74961523
CGCAAAG3150.015.71365212
ACGGTAG2302.5465852E-1115.65154413
CGAGCAT2600.015.57703835
Produced by FastQC (version 0.11.2)