FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell47.3510000009557c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell47.3510000009557c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582815
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA96670.26981577335140106No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC55560.15507359436644091No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG54800.15295235729447376No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT50680.1414530194832834No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA40440.11287214103993648No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT39550.11038806078460651No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT38920.10862966689600217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG38550.021.6530445
CCGATCT100350.021.120444
TCCGATC101350.020.9345423
TTCCGAT101500.020.8817272
CTTCCGA105100.020.4778941
CGATCTA15300.019.9993975
CGGTACC4900.019.28486645
CGATCTT24600.019.1152785
CGGTCGA1002.0491314E-517.99945820
CGATCTC29550.017.4360245
TTAACGG5300.017.40635135
ACGTCCG2350.017.23328443
CGATATA952.8223934E-416.57821745
GATCTCG12000.015.937026
ATCTGCG4450.015.6734687
TAACGGC6250.014.76058636
TTAGACG2751.8189894E-1214.727241539
GATCTGG19600.014.5786436
CGGTACT9200.014.18475238
CCCCTAT36250.014.0355871
Produced by FastQC (version 0.11.2)