FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell46.35100000095553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell46.35100000095553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38917
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.629544928951358No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.5575969370712028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.3854356707865457No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT1440.3700182439550839No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG1410.362309530539353No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT1150.29550068093635173No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA1000.25695711385769715No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.2081352622247347No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG640.16445255286892618Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT570.14646555489888738Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC530.1361872703445795No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCAC500.002225808622.57
CATCTCC550.003869615320.45454645
ATCTTTT7750.016.5483887
TCTTTTT7900.016.2341778
GATCTTT8000.016.031256
CGATCTT9150.015.4918035
CACATCT900.003667816315.045
CTTTTTT8550.014.9999999
ACACATC950.005253533414.21052644
GATCTCA1555.5340533E-513.0645166
GATCTCC1300.00293225412.1153856
GATCTCG1506.20582E-412.06
CTTATAC2452.0322477E-711.93877645
CGAGATC1350.00388943111.66666712
TAGGGAG1350.00388943111.6666674
CGATCTC4750.011.3684215
CCGATCT23750.011.1789474
TTCCGAT24550.010.9063142
TCCGATC24600.010.8841473
TCTTATA2707.1561044E-710.83333444
Produced by FastQC (version 0.11.2)