FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell45.35100000095539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell45.35100000095539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409033
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18600.13200542499714343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC35450.024.4970173
CGATCTG13500.024.1644175
CCGATCT35750.024.1026544
TTCCGAT35850.024.0981772
CTTCCGA36350.023.9668371
CGATCTT10100.021.6069015
CGATCTA6700.020.1473795
GCATTCG3200.018.98328222
TACGGTC600.0065147118.7482535
CGATCTC9850.018.7292215
CGCTAGA3000.018.0008837
CCGCTAG3300.017.04689436
ATTCGTA3650.016.6446524
TTAACGG1501.4220313E-716.50139235
ATCTGCG1855.629772E-915.80934057
GTATTAG5300.015.7155681
GATCTCG3600.015.6235466
TTCGTAT4050.015.00073325
ACCTCGA1502.5750924E-614.99966839
GCGCGAC900.0037559214.99913618
Produced by FastQC (version 0.11.2)