FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell44.3510000009551f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell44.3510000009551f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479736
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA11500.23971517668050762No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG6920.14424600196774892No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT6480.13507429085997297No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC6340.13215601914386246No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA5200.10839294945553388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG406.158944E-428.126537
GTCGGAA406.158944E-428.126539
GGTCGGC507.152336E-526.99862522
CGGTCGG507.152336E-526.99862521
CAGGTCG600.006503094518.75140
CGAACGT600.006507009718.7490444
TCGGCAT600.006507009718.74904424
AATAGCG851.203055E-418.5284676
CGATCTT5450.018.1642115
CGATCTG7050.017.5522985
GTATAAT2350.017.2457471
AGGCGGT1053.104894E-517.14377239
ATAGCGG800.001709466216.8741427
CCGATCT19050.016.6526954
GGTACTA1104.62635E-516.36450839
CTTGCGG1104.62635E-516.36450834
CCTTGCG1104.62635E-516.36450833
CGCGGTA1104.6304827E-516.36280343
TCCGATC19350.016.278243
ACCATCG1257.6112883E-616.19917318
Produced by FastQC (version 0.11.2)