FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell43.351000000954f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell43.351000000954f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678803
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA35960.13423906125235788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24550.024.3792255
TCCGATC57350.024.1285613
TTCCGAT57550.024.0838072
CTTCCGA58850.023.561031
CCGATCT58950.023.4736734
CGATCTA10200.020.9562285
ATATGCG550.003937506620.4548854
CGATCTT13650.020.1102245
CGGTACT4850.019.94878438
TTGCGGT5300.017.83048435
CGATCTC17300.017.8182145
GGTACTA5200.017.74067939
CCTTGCG5250.017.5717233
CGGGTAT1053.115976E-517.14218115
GTCGACG800.001712099516.87433443
CGCATCG3450.015.65155713
TAACGCC3550.015.2115194
GTATATA9100.015.0885831
GCGGTAC6300.015.00024937
CTTGCGG6500.014.53870334
Produced by FastQC (version 0.11.2)