FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell42.351000000954da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell42.351000000954da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207004
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG602.4683305E-422.50023840
CGATCTG25550.021.8392665
TCCGATC66400.021.0765533
TTCCGAT67000.020.8878082
CCGATCT67100.020.7560834
CGATCTA10000.020.699755
CTTCCGA68000.020.6277431
CGATCTT17150.018.6295135
CGGTACC1358.596344E-716.6657145
CGTCCGA850.002567151815.8814421
CGTCGTA3300.015.68091810
GTATTAA6300.015.3674031
GTCTAGC1209.703232E-515.010021
TATTAGA4550.014.8349862
GTATAGA3950.014.8200211
GCATTCG7150.014.78969522
CGATCTC21100.014.7154635
GGGGTTA2601.0913936E-1114.711366
CGCTTCG4900.014.694365532
GTCTAAT2152.561137E-914.6609511
Produced by FastQC (version 0.11.2)