FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell41.351000000954b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell41.351000000954b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979002
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22710.23197092549351278No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC13040.13319686783070925No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT11860.12114377703007757No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG10900.11133787264990265No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA9970.10183840278160822No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT9960.10173625794431473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG651.52400025E-524.2296416
CGGTACC1450.023.27477845
TTAACGG1650.021.81939535
CGATCTG14050.020.3371325
CCGATCT34250.020.1669454
TCCGATC34400.020.1444113
CTTCCGA35400.019.842761
TTCCGAT34950.019.8274042
GAATCGG807.5642405E-519.68658422
CGATCTT7800.019.0375755
GGCGTTA1507.2650437E-917.99916342
ATAGCGG1507.2650437E-917.9991637
CTAACGT750.0011104117.9991639
TACCGCC750.0011104117.9991638
CGATCTC9350.017.8066585
GTATATA2550.017.657961
GATCTGT4350.017.5853886
CGATCTA5250.017.142065
CGAGCCG1853.074092E-1017.02623415
GCGTTAT1601.6134436E-816.87421443
Produced by FastQC (version 0.11.2)