FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell40.35100000095497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell40.35100000095497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348058
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA32910.14015837768913714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA10400.021.8497415
CGATCTT16950.021.5032255
TCCGATC66350.021.2949683
CCGATCT67250.020.9765244
TTCCGAT67700.020.8375382
CTTCCGA69050.020.5044781
CGATCTG26400.019.260295
CGGTCGA1102.2592249E-618.40806420
CGGTACC3150.017.85614645
TCGTTAA3550.017.74548712
TTAACGG3100.017.42060735
GTATTAG8950.017.353531
TATTAGA5850.017.3070952
CGATCTC20200.015.81594855
GATCTCG7250.014.5853926
GGCGTTA3550.014.57727142
CTCGTTA4350.014.48194911
CGTCGTA2651.6370905E-1114.43315610
CCCCTAT12550.013.9898381
GTATATA7250.013.9712821
Produced by FastQC (version 0.11.2)