FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell4.35100000095007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell4.35100000095007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388398
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA41640.1743428021627886No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC25080.10500762435741447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT352.8114233E-432.162041
TTCCGAT45100.027.1887192
CTTCCGA46000.026.7714021
TCCGATC46200.026.5413683
CGATCTG18600.026.2491685
CCGATCT47400.025.9643694
CGATCTA7350.025.1012465
CGATCTT13750.021.7629495
CGATCTC13550.019.5934755
GTCTATA3500.018.6539821
ACAATCG1253.8371036E-718.00018537
CCTTGCG5700.017.76371233
TAATACG1551.0921212E-817.4188044
GGTACTA5700.017.36823539
TTGCGGT5850.017.30786935
CGGTACT5850.017.30750738
CGCGGAA800.001711217116.87552633
TACCGTC2159.094947E-1216.7436567
TCGAGTA1358.5928696E-716.66648941
TCGGTAA952.820731E-416.57911727
Produced by FastQC (version 0.11.2)