FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell39.3510000009547d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell39.3510000009547d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249702
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA32880.14615269044522342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24100.023.7134365
CGATCTA9400.023.6965525
CCGATCT64150.023.1135434
TCCGATC64400.023.0587563
CTTCCGA65500.022.686131
TTCCGAT65550.022.6198922
CGATCTT16400.021.8137875
CGATCTC19800.016.9316355
TACCGTC3200.016.8744437
TATCGAT1205.1791703E-616.87444142
CGTCGTA2900.016.29256410
TATCCCG1257.633462E-616.1998235
CGATTAC1257.635073E-616.19946545
ACCGTCG3200.016.171348
CCGATTG850.002566374115.88218136
CGGGTAT1451.8112241E-615.51672815
TAACGCC3050.015.4916344
GGCGTTA3050.015.49129142
ATACCGT4100.015.3656866
GTATATA6750.015.3430571
Produced by FastQC (version 0.11.2)