FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell38.35100000095454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell38.35100000095454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684195
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA79890.21684519956191245No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC47440.1287662569435114No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT41770.11337619208538093No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37440.10162328541241708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG56500.019.871255
CGATCTT31950.019.2953555
TCCGATC143350.018.834613
CCGATCT143150.018.8137724
TTCCGAT144800.018.6773342
CTTCCGA149250.018.3442061
CGGGTAT2100.018.21364215
CGGTACT10300.017.91223338
CGATCTA23100.017.8242875
TTGCGGT10600.017.4055235
CGGTACC5200.017.30708345
CGGTAAG1205.177739E-616.8755528
GGTACTA10800.016.87463439
CCTTGCG11300.016.7262133
CGACGTA1501.4253601E-716.49986526
ATCTGCG5600.016.4726337
GGCGTTA4650.016.45103342
TTAACGG5500.016.36350335
GTATTAG15300.016.1875441
GCGTATC850.00256720915.88179344
Produced by FastQC (version 0.11.2)