FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell37.3510000009543a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell37.3510000009543a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169854
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13940.11916016870481275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT10500.020.9993615
TCCGATC38200.020.6734543
CCGATCT38950.020.3331434
CGATCTG13100.020.266565
CTTCCGA39650.020.0979711
CGATCTA6500.020.0763115
TTCCGAT39550.019.9677872
ACCGTCC807.5649106E-519.6869038
CGAACTA901.8655369E-417.50021629
GATCTTT7850.017.196936
GCGACTC952.819143E-416.57915141
TCGTTTA1652.357774E-816.36383830
GTATTAA3150.015.7218721
GGCGTTA1451.8085593E-615.51743342
GCATTCG3050.015.49133222
GGGTTAG3200.015.468287
CGCGCTA1056.038365E-414.99954416
GGGGTTA3300.014.9995446
TATTAGA2900.014.74093152
GATCTAG3100.014.5156886
Produced by FastQC (version 0.11.2)