FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell36.35100000095410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell36.35100000095410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714581
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33500.12340762718076934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC602.468977E-422.4996413
TCCGATC67450.021.582313
TTCCGAT67700.021.4693782
CCGATCT68950.021.2106824
CGATCTG26450.021.0960715
CTTCCGA69850.021.046431
CGATCTA9900.020.454225
CGATCTT21050.019.2395975
GTATTAG10200.017.436471
CGATCTC17850.017.2686315
GGCGTTA3000.017.24940742
GCGTTAT3500.016.07087543
CGTTATT3250.015.92252944
CTAGCGC850.002566961315.8818069
TAACGCC3500.015.4283264
GTATTAA8600.015.18312551
ATCTGCG3300.014.9994857
ACGGGTA1056.0421915E-414.9994848
TCGTTTA3650.014.79482930
CCCCTAT15450.014.4257481
Produced by FastQC (version 0.11.2)