FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell35.351000000953e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell35.351000000953e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703770
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19030.11169347975372264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC38700.022.150243
CCGATCT39050.021.8940934
TTCCGAT39750.021.7349432
CTTCCGA40250.021.6434351
CGATCTG14900.021.1400595
CGATCTA5800.019.7836575
ATACCGT2650.019.5274876
TACCGTC2200.019.4310077
CGATCTT12650.019.0308275
CGTCGTA1901.8189894E-1218.94657710
CGATCTC10900.017.3387265
ACCGTCG2251.8189894E-1216.999298
GTATTAA5500.016.78041
GTCTATA2403.6379788E-1215.9447891
GTATTAG6400.015.8275491
CGGTACT2651.8189894E-1215.28282838
GGTACTA2850.014.99981439
TATACTG3650.014.7939035
TCTTCCG22300.014.1249715
GTCCTAT5500.013.9154521
Produced by FastQC (version 0.11.2)