FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell34.351000000953db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell34.351000000953db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674640
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA9470.1403711609154512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG4950.027.7275
CGATCTA2700.026.6664035
TTCCGAT15900.024.0563682
TCCGATC15850.023.99033
CTTCCGA16150.023.8338931
CCGATCT16000.023.6247654
TCGAACG951.3146278E-518.9471823
TATACTG1454.7621143E-918.6205045
CGAACGT1002.0447736E-517.999824
CGATCTT4950.017.7270985
TTGCGGT1153.4447166E-617.60852235
CTTAGGC1153.4447166E-617.6085223
GTAGGAC1053.1096053E-517.1426893
ACGTCTG1053.1096053E-517.1426897
TCGCCGT1205.167205E-616.87483436
CGCTTTC1904.474714E-1016.57878345
GGTACTA1104.6333415E-516.36347439
ACGTTCG1104.6333415E-516.36347444
CGTTCGA1104.6333415E-516.36347445
GAACGTC1104.6333415E-516.3634745
Produced by FastQC (version 0.11.2)