FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell33.351000000953b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell33.351000000953b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459124
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA702.7032085E-522.50046338
CGATCTT28400.018.6962875
TTCGTCG851.2045332E-418.52979337
CGTCGAG851.2045332E-418.52979339
TTAGTCG1205.1740153E-616.87534528
CCGTCCA1104.6408E-516.362859
GTCGCCT1401.2549935E-616.07175831
CCGATCT80900.015.9077664
GTTGACG850.002565256815.8826836
CGTCAAC850.002565256815.8826828
TCCGATC81600.015.7713023
AACCGCG2009.276846E-1015.75032441
TTCCGAT81500.015.7354412
GATCTTT26550.015.6772146
CTTCCGA84800.015.3187911
TTGCGGT900.003754054215.00030835
TACGACG1056.04044E-414.9992815
TAGACTA1654.2043212E-714.999285
GCGATCC900.003755766214.9992813
TGTCGCC900.003755766214.9992810
Produced by FastQC (version 0.11.2)