FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell32.35100000095398.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell32.35100000095398.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610689
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26450.1013142507590908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC65650.023.5791193
CCGATCT65850.023.370834
TTCCGAT67050.023.0867862
CGATCTA10850.023.0179735
CGATCTG24250.023.009855
CTTCCGA68500.022.8748761
CGATCTT19500.022.7303125
CGATCTC17900.016.8432395
GATCTAG5800.015.9048546
GATCTTT16650.015.6753636
TACGTGA1209.75819E-515.00027436
GGTACTA3300.014.31844439
ACAACGC1108.592311E-414.31844333
TCTATAC3950.014.2402223
TAATACG1758.181869E-714.1425744
TATACCT13900.014.0824535
CTATACC13700.013.9595754
GATCTCG7100.013.9433836
TTAACGG2752.910383E-1113.90934535
CAATCGA1301.9273066E-413.84640638
Produced by FastQC (version 0.11.2)