FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell31.3510000009537e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell31.3510000009537e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687222
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA20230.11990123409960278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA453.4925557E-529.99905241
TCGACGG507.1633374E-526.99834644
CGATCTG12700.024.0930125
TTCCGAT38250.023.763252
TCCGATC38050.023.6516423
CTTCCGA38700.023.5036491
CCGATCT38550.023.228154
CGATCTA5350.022.7088895
CGATCTT13250.021.7345185
GTCGACG654.241118E-420.76795843
CGTCGAC750.001110972417.998942
TATCCCG1153.4526183E-617.6076165
GCCGTCG800.001711655616.87446640
CGATCTC10950.016.8482845
TACCGTC2550.016.7636787
TCTATAC3100.016.6925263
GTATTAT3700.016.4295981
ACGACGG2750.016.3626356
GTATAGA2550.015.8926841
GTACGGG850.002554724715.8926841
Produced by FastQC (version 0.11.2)