FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell30.35100000095355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell30.35100000095355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903165
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC22050.023.3666843
TTCCGAT22050.023.2646472
CCGATCT22100.023.2120154
CTTCCGA22300.022.910551
CGATCTA3550.022.8162565
CGATCTG8600.022.7609835
CGATCTT6550.020.6101065
CGTCGTA706.9832115E-419.28516810
TACACCG706.9832115E-419.2851685
GGCGTTA951.3156432E-518.94683342
TACCGTC750.001110183517.999497
CGCGGTC2159.094947E-1216.74371144
AACCGCG1904.474714E-1016.57847841
CCCTAAT1501.4168108E-716.5050141
GTCTCGA1104.6368696E-516.36317411
GTCCTAT2403.6379788E-1215.9423441
ACCGTCG850.002565078715.8819038
GCGTTAT1156.781567E-515.651731543
CTAGGAC1301.106164E-515.5764823
CGATCTC6600.015.3404765
Produced by FastQC (version 0.11.2)