FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell3.35100000094fef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell3.35100000094fef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097673
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18050.16443877183824326No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC11760.10713573167965323No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT11430.10412937186211194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC401.5610654E-533.74822641
CGATCTA3650.027.121865
TCCGATC19800.026.475883
CGATCTG7550.026.2237855
CCGATCT19900.026.1167164
TTCCGAT20400.025.6971762
CTTCCGA20700.025.1233941
TACGGGT450.001226895724.9986844
TTCGCTA500.002264039822.50086435
ACGGGTA754.604257E-520.9988966
ATCGTAA654.238266E-420.76908539
AACCGTA654.2395692E-420.76813716
CGATCTT6350.020.55015
CTTACCG550.003937627720.4534683
GGTACCT5700.020.1305189
CGATCTC5500.019.635335
TCGTAAT706.985669E-419.28469840
TCTTCCG10150.019.0630365
ATCTACG951.3163402E-518.9463717
AGACGTA600.006508868718.75072324
Produced by FastQC (version 0.11.2)