FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell29.3510000009533b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell29.3510000009533b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830625
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA11130.13399548532731376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1156.7611836E-921.52202635
ACCGCCT1156.7666406E-921.52073112
TTAGGCT955.5271994E-721.3160744
GCGGTAA955.5271994E-721.31607434
CGATCTG7400.020.980015
GACTGCG550.003936720.4535887
TCCGATC20850.020.1801243
CTTCCGA21550.020.0575391
TTCCGAT21200.019.9530962
CCGATCT20950.019.8690014
CGGTACC1302.7710485E-819.03756945
CGATCTA3550.018.3805275
ATTAGGC1354.2671672E-818.3335783
TCGCCTA901.8647008E-417.50023333
TATTAGG1551.0884833E-817.4195882
GTATTAG3150.017.152381
GTATTAT1853.0377123E-1017.0364841
AAGCTCG2650.016.9813624
CGGTAAT1205.1689603E-616.87522735
GTACCTT800.001710152716.8752256
Produced by FastQC (version 0.11.2)