FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell28.35100000095311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell28.35100000095311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457579
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA34870.14188760564767194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC65050.023.0705173
TTCCGAT65750.022.9622482
CGATCTG25050.022.9039965
CCGATCT66000.022.7043514
CTTCCGA67050.022.561161
CGATCTT17100.021.9734955
CGATCTA12100.021.1981645
GTATTAG9600.017.3520741
CGATCTC17650.017.0820065
CGACCGT800.001711924716.87451219
GTATTAA6250.016.567951
TATACCG1401.2562305E-616.0712916
CGATTAC1855.629772E-915.81067645
TAGGCGG1301.1077174E-515.57647217
CGGTACC3050.015.4916745
GGTACTA4100.015.36572339
GTCGACG900.00375575714.9998743
GGGGTTA5850.014.2306476
GTATAAT9550.014.142911
CGATCGC5100.014.11723915
Produced by FastQC (version 0.11.2)