FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell27.351000000952f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell27.351000000952f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768007
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT27800.019.1001515
CGATCTG43200.019.0619355
TCCGATC104600.018.3049073
CCGATCT104250.018.3016154
TTCCGAT106000.018.0422422
CGATCTA14100.018.031385
CGTAATC750.001110847117.9997941
CTTCCGA109750.017.5996281
GTATTAG9300.017.1868441
CCGTCGA1053.115294E-517.14265841
CGTCGAC1257.634795E-616.19981242
GGGGTTA5200.016.0091386
CGTTAGG850.002566898215.8818819
ACACCGT3000.014.9995556
CGGGTAT1859.631913E-814.59416115
TTAACGG2801.8189894E-1214.4646435
GTCTATA4050.014.4523691
ATCTGCG5050.014.2570027
GGCGTTA4900.014.23452942
GGGTTAG6250.014.0395837
Produced by FastQC (version 0.11.2)