FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell26.351000000952dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell26.351000000952dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046543
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA40050.13146047831919655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG34850.021.7567235
TCCGATC87100.021.4917373
CCGATCT88300.021.2761044
CGATCTA13500.020.999225
GTCGACG754.607158E-520.99921843
CTTCCGA91050.020.9870281
TTCCGAT89650.020.9313092
CGATCTT22550.019.7553625
TAGCCGA1507.2814146E-917.999336
GGCGTTA3650.016.6434942
GTATTAG10950.016.4436821
CGATCTC25350.015.7094755
GCGGAAT1908.014467E-915.39441619
CCGAATA1553.623898E-614.5153519
CGGTACT5750.014.47819938
GTAGCAC2203.5779522E-914.317653
TACCGTC4400.014.3174137
GTATAAT9700.014.1540691
CCTTGCG6000.013.87607833
GCGTTAT4400.013.80630543
Produced by FastQC (version 0.11.2)