FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell24.35100000095299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell24.35100000095299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778162
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23690.1332274562160253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT957.4396667E-1026.05124715
CTTCCGA50950.022.7141251
TCCGATC51450.022.3901123
TTCCGAT51700.022.2818412
CCGATCT51600.022.1942144
CGATCTG18700.021.7775335
CGATCTT14800.021.739325
CGATCTA7500.020.9994755
GTATTAG7750.020.0458491
TACCGTC2300.019.5641787
TACGCGC706.984059E-419.28631628
ACCGTCG1901.8189894E-1218.9463628
CGATCTC14650.018.5831535
CGTCGTA1951.8189894E-1218.46055810
TCGGTAA800.00171098516.87552627
CCGGGTA1601.616172E-816.87410414
CCGTCGT2159.094947E-1216.7432989
TATCGCC1803.912646E-916.25050731
GTACGCG850.002565370415.88284927
ACGCGCT850.002565370415.88284929
Produced by FastQC (version 0.11.2)