FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell23.3510000009527f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell23.3510000009527f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92328
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.9119660341391561No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7660.8296508101550992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.5296334806342604No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.5198856251624643No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT3200.34659041677497615No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT2890.31301447014990036No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG2710.29351875920630793No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA2320.2512780521618577No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG1820.1971232995407677Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1610.1743783034399099Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAA1070.11589117060913266No Hit
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT960.10397712503249285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.10289402998007104No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG654.1857324E-420.77798835
CGATCCT654.1857324E-420.77798834
ATCCTGT654.201092E-420.76673136
TCCCGGC654.201092E-420.76673120
GATCTTT15300.019.9975936
CATCTCC807.474166E-519.68513145
ATCTTTT15850.019.161737
TCTTTTT16200.018.7477448
ACCCCCC1002.015795E-517.99783341
CTTTTTT16950.017.9181969
CGATCTT18350.017.8997525
CCGATCC800.001690021716.88211333
TCCTGTC952.786844E-416.57695237
ACATCTC1108.4911345E-414.31645844
TCCCCGT1000.00748808113.4983755
CACATCT1454.7789715E-412.41229843
GTCTCGG2301.3123718E-611.73771717
CTTATAC3307.3250703E-910.90777845
GATCTCT2503.575924E-610.79869925
CGTCTCG2503.575924E-610.79869916
Produced by FastQC (version 0.11.2)