FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell22.35100000095249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell22.35100000095249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737700
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA16710.22651484343228956No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG10230.13867425782838552No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC9190.12457638606479599No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT8300.11251186119018572No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT7830.10614070760471736No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA7550.10234512674528941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG250.002106309236.00108333
CGATCTA3050.025.820455
CGGACGT500.00226345122.50067736
ATCGTAC654.237326E-420.76844825
GACATCG654.237326E-420.76844822
TCCGATC20800.020.5535033
CGACACG550.003934535720.45516236
TCGTTAA550.003936080720.45377512
CGTCGTA550.003936080720.45377510
ACCGTCG550.003936080720.4537758
CCGATCT20850.020.3962974
TTCCGAT21150.020.2133732
GCTCTAG1351.9535946E-920.0060271
CCGATCG908.247358E-619.99924718
CGATCTT4900.019.7454935
CTTCCGA22650.019.4760651
GATCGCA1051.4446559E-619.28498820
CGATCTG8300.019.2475665
GGGGTTA2700.019.1672426
GCGACGA951.31444685E-518.94793941
Produced by FastQC (version 0.11.2)