FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell21.3510000009523c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell21.3510000009523c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918357
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36160.12390533440562618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC88050.021.975333
CGATCTG31150.021.9574475
CCGATCT88500.021.8127464
TTCCGAT89200.021.7424622
CTTCCGA89650.021.5953431
CGATCTA13600.020.6793755
CGATCTT25250.019.781445
CGAGCGT1603.8198777E-1119.6867666
TTAGCGT807.571258E-519.68642821
CGATCTC26550.017.7959465
ATCTGCG3700.017.6341747
CGGGTAT1501.4258148E-716.49910215
TTAGCGA1156.783438E-515.65346727
GTATTAG10150.015.3036491
GATCTCG9600.014.9994416
CGGTCGA1806.888149E-814.99918420
TAGCGTT1056.0433033E-414.99918322
CCGTCGG4000.014.62470540
ACAATCG1402.232355E-514.46424237
GTGCGTA1402.232355E-514.46424238
Produced by FastQC (version 0.11.2)