FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell20.35100000095212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell20.35100000095212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681675
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC62100.025.4339623
TTCCGAT62800.025.1146352
CGATCTG24850.025.0796725
CCGATCT63900.024.6470894
CGATCTA10600.024.6218475
CTTCCGA64400.024.4707891
TAACGCT550.003938622320.4538844
CGATCTC17350.020.1002145
CGATCTT18050.019.3207045
CGACGTA1102.2577515E-618.40952734
ACGACGT1451.8104711E-615.51760933
GTATTAA7400.015.5158031
TACGACG5850.015.3841185
GGCGTTA3600.014.99951542
GTATAAT7700.014.9112911
CGCGGTT7850.014.90397710
GATCTCG8400.014.7316666
GATCTGT11650.014.6776376
ACGACGG6300.014.2852536
TCGAGTA950.0053796414.21006741
Produced by FastQC (version 0.11.2)