FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell2.35100000094fc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell2.35100000094fc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075047
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA38480.1854415827689686No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT23980.11556364747400902No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC21810.10510605301952197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC34750.026.2218993
CCGATCT35300.025.7496054
CTTCCGA35850.025.5600831
TTCCGAT36000.025.3114132
CGATCTG14700.025.254025
CGATCTA5500.024.9534785
TACCGTC1201.6370905E-1124.3739557
CGTCGTA1301.2296368E-920.76834110
ACGGGTA1351.9681465E-919.9991446
CGATCTT11500.019.7600255
GGTACCT11600.019.3957219
GGTACGG706.9564104E-419.2979091
ATACCGT1757.2759576E-1219.2848876
CGGTACT4800.018.75010138
ACCGTCG1851.6370905E-1118.2424628
TATCCCG901.867202E-417.499255
TTGCGGT5150.017.4758235
CGATCTC11650.017.381235
CGGGTAC3050.016.9664867
TACCTGG13350.016.8532111
Produced by FastQC (version 0.11.2)