FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell19.351000000951f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell19.351000000951f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722066
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21360.12403705781311519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG13350.027.3007625
TTCCGAT31550.027.0972542
CTTCCGA32200.026.5633721
TCCGATC32700.026.2818953
CCGATCT33850.025.3225444
CGATCTA4400.024.0317965
CGATCTT9650.020.9824525
CGATCTC10900.017.1313925
TTAACGG1752.6884663E-916.71608735
GTATTAG5800.015.9115081
CCCAACG850.002566502415.88175821
CGGTACC2051.3205863E-915.364387545
TCTTCCG15300.014.9985695
TAACCGT1056.0434034E-414.9985677
GATCTGG9500.014.9196276
GATCTGC7250.014.8951296
ACGCTAT4000.014.62360410
GATCTGT6400.014.4126856
GTGTATA2509.640644E-1114.4057351
GTATATA6750.014.3390431
Produced by FastQC (version 0.11.2)