FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell18.351000000951dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell18.351000000951dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246457
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA84410.2600065240352791No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC51000.15709433391540378No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT45240.13935191502613464No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG42600.13121997303521962No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT40760.12555225589003643No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA38910.11985373593428159No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA32630.10050957089528677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG38600.022.208075
CCGATCT110250.021.0403634
TCCGATC110750.020.9250563
CGATCTT31200.020.8408975
CTTCCGA111800.020.8403911
TTCCGAT111400.020.7625682
TTGCGGT10250.019.53676635
CGATCTA17300.017.947595
GCTACGC2800.016.87463617
CGATCTC30300.016.7818165
CCGGGTA2301.8189894E-1216.63007714
TTCGTCG952.821476E-416.57884637
TCGTCGA952.821476E-416.57884638
CGAGCTC1803.918103E-916.2496510
TCGGTTA3100.015.9678933
GATCTTT25950.015.9534126
GGGGTTA17000.015.882016
GGTACTA12300.015.73160939
TATCGCC8450.015.71020431
TCGCCTA8500.015.61779133
Produced by FastQC (version 0.11.2)