FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell17.351000000951a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell17.351000000951a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247593
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG27350.025.1738035
TCCGATC63450.024.8228233
TTCCGAT64050.024.555542
CTTCCGA64550.024.450861
CCGATCT65050.024.2122694
CGATCTA10550.021.9669385
CGATCTT17050.019.9267885
TAGACCG750.001110893717.99981527
CGATCTC19050.017.1260725
CGGGTAT1652.363413E-816.36321615
GTATTAG10950.016.0380971
TACCGTC3400.015.8819467
ATCTGCG3450.015.65177257
TTAACGG2950.015.25408135
ATAGTCG1056.040831E-415.0000776
ATAGGGG8850.014.7458383
TAGGGGT8250.014.454624
GGGGTTA7250.013.965596
CGATATT1453.1085427E-513.96515914
CGTCGTA3450.013.69530110
Produced by FastQC (version 0.11.2)