FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell16.3510000009518d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell16.3510000009518d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452449
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15950.10981452705051951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG300.00514818229.9994265
TACCGTC1551.8189894E-1221.7737777
TCCGATC37600.021.4823023
CGATCTG14000.021.3745925
CTTCCGA37850.021.3514391
TTCCGAT37750.021.3380762
CCGATCT38050.021.1099784
TAGAACG654.2395186E-420.7688334
CGATCTA5400.020.4162775
CGATCTT11900.019.0962735
GTCTATA2100.018.2233471
TCGGACT750.001110557217.9996574
ACCGTCG1406.480877E-817.6782348
CGGTACC1459.674659E-817.06863845
CGATCTC10500.016.4996835
GGCGTTA1652.3599569E-816.36332342
ATACCGT2101.07320375E-1016.0711216
TAGTACC6250.015.8396984
TTAGGAC2850.015.7891723
AGTACCG5500.015.54515655
Produced by FastQC (version 0.11.2)