FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell15.35100000095163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell15.35100000095163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658548
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA61810.2324953320383909No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG36660.1378948207818704No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC35860.1348856593900129No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT32000.1203664556743004No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA28680.10787843589809175No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT28130.10580963744118971No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT26760.10065644855763371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC654.240918E-420.76874542
CGATCTG36300.019.8962295
CCGATCT94350.018.8628334
TTCCGAT94550.018.7756942
TCCGATC94800.018.7495613
TCGACGG600.006513572318.74956144
CGATCTT22850.018.4131365
CTTCCGA100400.017.9179271
CCCAACG2350.017.23363921
CGGTACT7050.017.23363938
GTATTAG13650.017.1543941
CGGTACC5850.016.9226845
CGGTAAG800.001711480616.8752428
CGCCTAT3900.016.73100734
CGATCTA15750.016.7138945
TACGCAA2500.016.19962110
ACTACGC2950.016.0165738
ACCGCCT6500.015.92270412
CGGGCTA2550.015.88198241
CGCGGTA6350.015.59018643
Produced by FastQC (version 0.11.2)