FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell14.35100000095157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell14.35100000095157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450465
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33430.23047781228778358No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG19650.135473796334279No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC18740.1291999462241419No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT16420.11320507561368251No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT15780.10879269751424543No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT14930.10293250785093055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC2500.025.19934545
CGCGGTA2850.021.31523343
CGCCTAG754.6046254E-520.99945316
TTAACGG2900.020.94845235
TAACGGC3100.019.5969436
ACGGATG706.9859024E-419.28521218
CGATCTT15750.019.1423575
CGGTCCG600.00651227818.74951439
AATCGGG1951.8189894E-1218.46169323
GGCGTTA1102.2573713E-618.40861142
TATACCG1354.2757165E-818.3328556
GGCACCG3200.018.2807759
CGATTGT1002.047695E-517.9995339
CCGCCTA750.001110608217.9995315
AACGGCC3400.017.86718237
CGATCTG23900.017.6982845
CCGATCT61800.017.293244
TCCGATC62000.017.2374553
TTCCGAT62050.017.2247522
TACGACG1053.1140276E-517.1424116
Produced by FastQC (version 0.11.2)