FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell13.35100000095120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell13.35100000095120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322016
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.617360628043327No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.48848504422140515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14990.4655048196362914No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.3937692537016794No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT5850.1816679916525887No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG5760.1788730994733181No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT4940.15340852628440824Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG4840.15030309052966312Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT4510.14005515253900427No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA3840.11924873298221207No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACA3570.11086405644440027No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTC3420.10620590281228263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCT250.002105040235.999389
TACTGGT250.002105040235.999382
ATTAATC300.005142254329.9994813
AAGCCAA300.005142254329.99948122
TGCGCGC300.005142254329.99948136
CCAACCT300.005142254329.99948125
CCCGCGA300.005142254329.99948136
TCGCTCG702.8730938E-828.92807220
TTCGCTC709.274099E-725.7138419
GCTGCTT450.001224760524.99956913
TGGTCTG551.3653775E-424.5450310
CGCTCGC751.6915801E-623.99958821
CTGGTCT602.4624486E-422.499619
AGACATC702.695554E-522.4996142
GACATCT702.695554E-522.4996143
ACTTAAG955.5071723E-721.31542230
CAGACAT754.5900408E-520.9996441
AGACCCC654.229601E-420.76887143
ACATCTA1301.2187229E-920.76887139
ATCCTCC550.003930873720.45419316
Produced by FastQC (version 0.11.2)