FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell12.35100000095106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell12.35100000095106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839518
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACAG12880.15342136797543351No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTC10120.12054536055212633No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG9660.11506602598157513No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA8830.10517940056079798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCAA450.001226373624.999675
GATCTTT22500.021.2997176
TCTAATG754.6012057E-520.9997222
CGATCTT27950.020.6884575
ATCTTTT22400.020.4907997
CTCTAGG550.003922409420.4664651
ATGTCGC550.003935968520.45427537
GCACTAT550.003935968520.45427513
GATCGTC1009.050091E-720.2497337
CTGTCCG807.560669E-519.6872399
TCTAGGG807.560669E-519.6872392
TCTTTTT23300.019.506188
ATATTAT1051.4365796E-619.2969531
TACACTT706.9819466E-419.285465
CTATTAG951.3085508E-518.958411
AGCGTCA1901.8189894E-1218.94711937
GTACTAG4650.018.8819641
TTAATAC1454.7675712E-918.6204433
TATTATA851.2040719E-418.5291672
CTTTTTT24900.018.252779
Produced by FastQC (version 0.11.2)