FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell10.351000000950c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell10.351000000950c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85091
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.5523498372330798No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.4101491344560529No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.3126065036255303No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.22093993489323197No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG1850.2174142976342974Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG1640.19273483682175555No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1620.19038441198246583Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT1520.1786322877860173No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT1390.16335452633063427No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA1140.133974215839513No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA1070.12574772890199903No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAA970.11399560470555052No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG950.11164517986626084No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAA880.10341869292874686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTA550.003908579720.45320926
TGCAGGG706.886657E-419.29582
GATCTTT16100.019.0049696
AAGCCCC600.006464631818.74877543
ATCTTTT16300.018.633757
TCTTTTT17150.017.7102138
CGATCTT19200.017.4597975
CTTTTTT17800.017.0634929
TTAGGGA800.001694063416.8738983
CATCTCC1104.5594337E-516.36256845
CTTATAC3700.014.59364145
GCAGGGA1757.9211895E-714.1419333
TCTTATA4150.013.01119744
GATCTAT1250.00222309312.5991766
ACATCTC1506.344954E-411.99921644
ATCTGCC2901.2763849E-811.6371717
CACATCT1558.3561166E-411.61214443
TAGACGT1751.7682934E-411.5706734
CTCTTAT4750.011.36767943
TACACAT1600.001090216311.24926641
Produced by FastQC (version 0.11.2)