FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell1.35100000094fac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell1.35100000094fac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231355
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36960.16563926403463367No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT23540.10549643602205834No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC22380.10029780111188046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6250.028.4386275
CGATCTG16550.027.5968535
TCCGATC44600.025.576123
CCGATCT45150.025.3642254
TTCCGAT45500.025.2191282
CTTCCGA45800.025.2154391
CGACGCC602.4693264E-422.49891344
GGTACCT16650.019.9990359
CGGTACC3300.019.77177245
TTAACGG3250.019.38541835
GTATTAG9750.018.4714091
TCTTCCG22850.018.215755
GTTGATC18250.017.87584917
CGATCTC14850.017.7264185
TACCTGG19050.017.36136411
GCCAGTA19000.017.29018826
GGGTACC19600.017.2185578
ACCGCCT3850.016.94723512
AGTAGCA19550.016.91885829
GCTAGAC800.0017120816.8741863
Produced by FastQC (version 0.11.2)