FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_H1_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_H1_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT240.24249772658381327No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG220.22228958270182883No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT220.22228958270182883No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG220.22228958270182883No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC200.2020814388198444No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG190.19197736687885217No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA170.17176922299686773No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT120.12124886329190664No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT120.12124886329190664No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC110.11114479135091442No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG100.1010407194099222No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG100.1010407194099222No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT100.1010407194099222No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA100.1010407194099222No Hit
ACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAA100.1010407194099222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)