FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G9_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G9_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336710
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA5670.1683941670874046No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA5430.1612663716551335No Hit
GTGCAGGCCCACTCATCTCCATCAGGTCATCTTTTAATTCCTCTGATGTG4840.1437438745508004No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4750.14107095126369873No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC4130.12265747973033174No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG3960.11760862463247304No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3870.11493570134537137No Hit
GGCCTGCACTGAGGAAGAAGGCAGAAGACGGAGAACTGTCAGGAAACAAA3830.11374773543999286No Hit
CTTTTATAACATGCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAA3690.10958985477116806No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT3560.10572896557868788No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG3410.10127409343351845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)