FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G2_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G2_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556605
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA451171.2685412071343316No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG213140.5992793689487588No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG189800.5336549883948316No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC133000.373952125692901No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG114580.32216116211949314No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACCTGTCTCT103150.2900237726708476No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC102680.2887022877153915No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA94300.2651404921266207No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA92100.25895481786703894No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGT91570.2574646327045033No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT87150.24503705078297985No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT87000.24461530026528105No Hit
GGGCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT86710.24379991593106348No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCT86520.24326569860864505No Hit
CCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCA85830.24132564622723077No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA84300.23702379094670337No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG84130.2365458070266448No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT82620.23230018514847728No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG82600.23224395174611745No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA81750.22985403214582445No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA80900.2274641125455315No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATCTG79930.2247367925310795No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT78850.22170018880364845No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA77020.21655483248772356No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT74100.20834475574318767No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT73260.2059829528440746No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG72360.20345244973788207No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA72140.20283388231192387No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG72120.20277764890956404No Hit
CTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGC70560.19839144352549695No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGCCA69390.1951017894874466No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA68970.19392088803789007No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTG68960.19389277133671015No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGG68150.19161531854113684No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACA66630.1873415799617894No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAAT66310.18644184552403206No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT66210.18616067851223286No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT65630.18452990984379766No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC65410.18391134241783946No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC64740.182027523438785No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG63750.1792439700219732No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG63050.177275800939379No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG62630.17609489948982246No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG60850.17109012667979717No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG59680.16780047264174683No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA59350.16687262150280954No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG58710.16507315262729486No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC55470.15596334144500162No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC55140.15503549030606434No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT55120.1549792569037045No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA54050.15197076987745337No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG52720.14823124862052436No Hit
GCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGGCTAACAG51810.14567262881315185No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG51230.14404186014471665No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA50970.14331082591403882No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT50760.14272037518926053No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG50640.14238297477510153No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATCTGTCTCTTATA50540.14210180776330236No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT50250.14128642342908476No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG50140.14097713971610568No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACCTGTCTCTTATACA49290.13858722011581268No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT49220.13839040320755328No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT49220.13839040320755328No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTTATACA48620.13670340113675822No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA47830.13448218174354476No Hit
GGATACACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAAT47580.13377926421404682No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA47450.1334137470987079No Hit
GGGCCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTC47000.13214849554561162No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA46240.1300116262559379No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT45800.12877449140402153No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG45500.12793099036862401No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC45390.12762170665564493No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG44720.12573788767659047No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC44340.1246694530317536No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA44100.12399465220343558No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA43360.1219140163161217No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA43260.12163284930432253No Hit
GCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTT43000.12090181507364466No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC42960.120789348268925No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT42930.12070499816538525No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA42920.12067688146420533No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATCTGTCTCTT42480.11943974661228897No Hit
AGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTAT42340.11904611279577011No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG42210.1186805956804312No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA41990.11806202825447301No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCTTATACA41890.11778086124267384No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGT41860.1176965111391341No Hit
AGATTGCTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTC39290.11047051893589534No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC39000.10965513460167772No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATCTG38800.10909280057807937No Hit
CAGAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG37930.10664664757542656No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA37900.10656229747188681No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA37770.10619678035654789No Hit
CACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAATTTTTC36870.10366627725035532No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA36120.10155752466186152No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT36000.1012201242477025No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACACAT35630.10017980630404558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)