FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G1_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_G1_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055149
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1974046.461354258008366No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1089163.564997975548819No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC231260.7569516249452972No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT216970.7101781287917546No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC203250.6652703354239023No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT193740.6341425573679058No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT179170.5864525756354272No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC177220.5800699082106962No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT162630.5323144632225794No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC151870.4970952316891909No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC149440.48914144612914134No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG126080.4126803635436439No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTGA118420.3876079366341871No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC107410.35157041440532033No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT99810.32669437726277833No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC93720.30676081592092563No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT88920.29104963456774124No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC86910.2844705773760952No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT82890.27131246299280326No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC77680.2542592848990344No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG77260.25288455653063074No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT76530.25049514769983394No Hit
CACACACACACACACACACACACACACCTGTCTCTTATACACATCTGACG75370.2466982788728144No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC68600.22453896683926056No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG57580.18846871298257467No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC56470.18483550229465076No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG55110.1803840009112485No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGA53450.17495055069327226No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC52840.17295392139630505No Hit
CACACACACACACACACACACACACCTGTCTCTTATACACATCTGACGCT48340.15822468887769467No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT48290.158061030738599No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC38470.12591857222020922No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA38340.12549306105856048No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA37960.12424925920143338No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC37270.12199077688191313No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA36810.12048512200223296No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA36420.11920858851728672No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT36150.11832483456617009No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA34700.11357874853239565No Hit
CACACACACACACACACACACACCTGTCTCTTATACACATCTGACGCTGC34540.11305504248728948No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA33850.11079656016776923No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACG33800.11063290202867357No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA32770.10726154436330274No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG32580.10663964343473918No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA32340.10585408436707996No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA31000.10146804623931598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)