FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F9_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F9_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296431
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA5180.17474555630146643No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA4500.15180598520397665No Hit
CTTTTATAACATGCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAA3950.13325192034571282No Hit
GTGCAGGCCCACTCATCTCCATCAGGTCATCTTTTAATTCCTCTGATGTG3610.12178213479696794No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT3590.12110744152939469No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG3250.10963765598064981No Hit
CATATATACAGTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAA3160.10660153627657026No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT3030.10221603003734428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)