FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544444
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA449841.2691412249706866No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG212630.5998966269462855No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG189390.5343292206055449No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC132720.37444518801820537No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG114410.32278687433064257No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACCTGTCTCT102850.29017245017836363No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC102450.2890439233910876No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA94030.2652884345189259No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA91890.25925081620699886No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGT91280.25752981285640286No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT86980.24539814989318495No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT86730.24469282065113737No Hit
GGGCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT86540.24415677042718123No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCT86110.24294360413085947No Hit
CCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCA85550.24136366662867292No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA84010.23701883849766No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG83950.23684955947956857No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG82450.23261758402728325No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT82410.23250473134855568No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA81540.23005018558623017No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA80730.22776491884199612No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATCTG79490.22426648580144024No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT78500.22147338200293193No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA76760.2165642904782809No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT73930.20857996345830265No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT73090.20621005720502286No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG72100.2034169534065145No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA71960.2030219690309679No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG71940.2029655426916041No Hit
CTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGC70270.19825394335472643No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGCCA69200.19523513419876293No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA68800.19410660741148683No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTG68770.1940219679024411No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGG67880.19151099580075182No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACA66420.1873918730271941No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAAT66080.18643262525800944No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT66070.18640441208832753No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT65490.1847680482467772No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC65140.18378058730791064No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC64620.18231350248445174No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG63630.1795203986859434No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG62830.17726334511139122No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG62490.17630409734220656No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG60730.17133857947819178No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG59380.167529801571135No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA59170.16693732500781505No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG58630.16541381384499232No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC55320.15607525468028272No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC55020.15522885958982566No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT54960.15505958057173425No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA53920.15212541092481643No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG52610.14842948569648723No Hit
GCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGGCTAACAG51630.14566459506766083No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG51110.1441975102442019No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA50820.14337932832342676No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT50680.14298434394788012No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG50410.14222258836646878No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATCTGTCTCTTATA50360.14208152251805925No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT50130.14143261961537548No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG50030.14115048791855647No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACCTGTCTCTTATACA49170.1387241553259129No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT49070.13844202362909389No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT49050.13838559728973007No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTTATACA48450.13669280710881593No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA47730.134661458891719No Hit
GGATACACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAAT47440.1338432769709438No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA47340.1335611452741248No Hit
GGGCCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTC46880.1322633394687573No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA46140.13017556491229654No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT45720.12899061178565666No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG45450.1282288562042453No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC45220.12757995330156152No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG44630.1259153762903293No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC44190.12467399682432562No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA43940.12396866758227806No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA43250.1220219588742268No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA43210.12190910619549922No Hit
GCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTT42900.12103449793536025No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC42850.12089343208695072No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT42800.12075236623854121No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA42780.12069593989917742No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATCTGTCTCTT42410.11965205262094702No Hit
AGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTAT42230.1191442155666728No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG42140.11889029703953569No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA41880.11815675462780621No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGT41740.1177617702522596No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCTTATACA41730.11773355708257768No Hit
AGATTGCTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTC39150.11045455930464693No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC38930.10983386957164509No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATCTG38680.10912854032959754No Hit
CAGAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG37850.10678684724599966No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA37820.10670220773695395No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA37630.10616615751299781No Hit
CACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAATTTTTC36730.10362697224162662No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA36050.10170847670325725No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT35890.10125706598834684No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACACAT35580.10038245772820786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)