FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F1_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_F1_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048869
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1971836.467414638018229No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1087853.568044412534616No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC230980.7575924055772812No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT216700.7107553653502331No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC203000.6658206698943115No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT193480.6345959764096128No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT178890.5867421657014453No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC176950.5803791504325047No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT162330.532426942581003No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC151710.4975943538407193No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC149250.4895257880873203No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG125910.41297281057336344No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTGA118230.38778314187982493No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC107190.35157299313286333No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT99700.3270065063471077No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC93560.3068678910113881No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT88740.2910587499823705No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC86750.2845317394745396No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT82720.2713137232199875No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC77580.2544550126620724No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG77110.2529134574165043No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT76310.2502895335942607No Hit
CACACACACACACACACACACACACACCTGTCTCTTATACACATCTGACG75250.24681283452978792No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC68480.22460787918405153No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG57510.18862732377153626No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC56390.18495383042039523No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG54990.18036196373146895No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGA53360.17501571894364762No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC52830.17327736941141125No Hit
CACACACACACACACACACACACACCTGTCTCTTATACACATCTGACGCT48210.1581242093379545No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT48190.15805861124239842No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC38420.12601394156324855No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA38280.1255547548943559No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA37930.12440678822212432No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC37230.12211085487766118No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA36730.12047090248875894No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA36330.11915894057763715No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT36030.1181749691442958No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA34650.1136487005509256No Hit
CACACACACACACACACACACACCTGTCTCTTATACACATCTGACGCTGC34470.11305831769092078No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA33800.1108607814897918No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACG33740.11066398720312352No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA32710.10728568528198489No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG32510.10662970432642399No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA32280.10587532622752896No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA30900.10134905763415876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)