FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_E5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_E5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40.2526847757422615No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.2526847757422615No Hit
GTCATGGGATGGAGACCCATGGCAGGCTCCCTACTCAGCCAGGAGTCTAC30.18951358180669614No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA30.18951358180669614No Hit
CTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTCTCTGTCTCTTATACA30.18951358180669614No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.18951358180669614No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.12634238787113075No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.12634238787113075No Hit
CACATGCACTGAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTG20.12634238787113075No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT20.12634238787113075No Hit
AAAGCAGACTCCCTGCTAGGCAAGGAACCTGATTGGGGACTTGATCCTGT20.12634238787113075No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT20.12634238787113075No Hit
CTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCA20.12634238787113075No Hit
GACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCTC20.12634238787113075No Hit
CACCCTTTGGTGTCCATGTCCTCTCCCTTTGGCGCTTAGGAGGAGATCTC20.12634238787113075No Hit
CCGTAACAGTCTCTGTGCGTGATTTCAGTCCTTTGAAATTTGCCTGTCTC20.12634238787113075No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA20.12634238787113075No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG20.12634238787113075No Hit
TCCTGGGATCGAGCCCTGCATCAAGCTCCCTGCTCAGTGGGAAGCTTGCT20.12634238787113075No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT20.12634238787113075No Hit
AGAAACAAGGAAGAACATTCAAGATAGAGGGAAGTATGAACAAAGACCTA20.12634238787113075No Hit
GCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATCTG20.12634238787113075No Hit
GNTCTGGGATCCCAGGACCCTGGGATCATGACCTGAGCTGAAGNCAGACG20.12634238787113075No Hit
CTTCCCTAGGTTGTCAGGAAGGGAGAAATAAGTACAACCCAGGACTTGAG20.12634238787113075No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT20.12634238787113075No Hit
CATGCTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCCTGTC20.12634238787113075No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT20.12634238787113075No Hit
AAGCACATGAGGGCAGAGAAAAAACTCTGATAAGCTGTGTTATACGCTAC20.12634238787113075No Hit
GCGTTGGGCTTTCTGCTCAGCAGGGAGCCTGCTTCTCCCTCTACCTCTAC20.12634238787113075No Hit
GCTCAGGTCATGATGCCAGGGATCTGGGGCGGAGCTCCGCACTGGGCTCC20.12634238787113075No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA20.12634238787113075No Hit
GAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGCTGTCTCTTAT20.12634238787113075No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGATATGGCCC20.12634238787113075No Hit
AGGCAGATCAGGCCTAGGGGGAAGCAGGCTCCCTGGTGAGCAAGGAGCCC20.12634238787113075No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCCTGTCTCTTATACACATC20.12634238787113075No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC20.12634238787113075No Hit
GAGCGAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGACTGTCTCTTAT20.12634238787113075No Hit
CTTGTGGGCTCTGGCCTGAATATGATGCGTTTTAACTAGGTGTGCTCTGG20.12634238787113075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)