FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT40.24330900243309003No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30.18248175182481752No Hit
GTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAG20.12165450121654502No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC20.12165450121654502No Hit
GCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCATATCTGTCTCTT20.12165450121654502No Hit
GTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAG20.12165450121654502No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA20.12165450121654502No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA20.12165450121654502No Hit
CCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGT20.12165450121654502No Hit
TCCTCAGGTAGTGGTCCATCAATCGGGTTGAGCTGGGGTGCACCAGTCTC20.12165450121654502No Hit
ATTGGTGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCCTGTCTCTTATA20.12165450121654502No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT20.12165450121654502No Hit
CTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACACAC20.12165450121654502No Hit
AGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTT20.12165450121654502No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT20.12165450121654502No Hit
GAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGTTGC20.12165450121654502No Hit
GATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGCT20.12165450121654502No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.12165450121654502No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.12165450121654502No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT20.12165450121654502No Hit
AAAGGAAACAGTCTACCTGAAAGCTTGACATGGTGTTGGAGCTGTCTCTT20.12165450121654502No Hit
ATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAAC20.12165450121654502No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGG20.12165450121654502No Hit
TATCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTG20.12165450121654502No Hit
CTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATCTGTCT20.12165450121654502No Hit
ATCCAGTCCTGTTAGTTCTGGATGCTGAACAAAACCTGTCTCTTATACAC20.12165450121654502No Hit
GTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGCTGTCTCTT20.12165450121654502No Hit
AATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGTCTAC20.12165450121654502No Hit
TTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC20.12165450121654502No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATCTGTCTCTTATACACATC20.12165450121654502No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20.12165450121654502No Hit
ATCAAGGAATGGGGCATCACCCAATCCATTGTCTGCAAATCGCTTGCGGA20.12165450121654502No Hit
GTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAATTCCC20.12165450121654502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)